Cytochemistry of Malaria under Treatment.
Abstract
The characterization of the nucleus of the erythrocytic stages of P. berghei and P. gallinaceum was aided by using pyrimethamine administration and DNAase or EDTA extraction. Studies combining these methods were particularly useful for the study of nuclear division of these parasites. During nuclear division, centriolar plaques, nuclear microtubules and electron dense bars located on the nuclear microtubules become apparent. They were not extracted by DNAase or EDTA. Because of their location and inability to react with DNAase or EDT treatment, the electron dense bars on the nuclear microtubules are suggested to be kinetochores. Ill-defined electron dense materials were noted at the equatorial region of the metaphase nucleus and were extracted by DNAase and EDTA treatments. It is suggested that they may be the poorly organized chromosomes of malarial parasites. The nuclear microtubules gradually disappear in the telophase nucleus. Resulting daughter nuclei eventually repeat the process of nuclear division. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1972
- Accession Number
- AD0745403
Entities
People
- Masamichi Aikawa
Organizations
- Case Western Reserve University