'Pseudomonas aeruginosa' Exotoxin: Effect on Cell Cultures

Abstract

An exotoxin, toxic to both mice and cultured cells, was isolated from cultures of Pseudomonas aeruginosa. Relatively small amounts of the exotoxin inhibited the uptake of uridine and amino acids by Vero cells. Within limits, this toxic action was reversible and could be inactivated by heating at 70 C or by proteolytic digestion, but it was not affected by nucleases. The inhibitory activity of the toxin could also be decreased by rabbit antiserum. Metabolic activities of Vero cells, generally associated with the production of energy, did not appear to be affected by the toxin.

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Document Details

Document Type
Technical Report
Publication Date
Jan 31, 1972
Accession Number
AD0745659

Entities

People

  • Francis B. Gordon
  • Olgerts R. Pavlovskis

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Animals
  • Biological Toxins
  • Biomedical Research
  • Cell Count
  • Cell Line
  • Cells
  • Culture Techniques
  • Cultured Cells
  • Endotoxins
  • Indicator Dyes
  • Lactic Acid
  • Materials
  • Metabolism
  • Tissue Culture
  • Toxic Actions

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).