Changes in Red Cells Following Rapid Freezing with Extracellular Cryoprotective Agents,
Abstract
The recovery of human red cells following slow freezing requires the presence of a high concentration of a penetrating cryoprotective agent. However, if the freezing rate is relatively rapid a variety of nonpenetrating compounds will provide good cryoprotection at low concentrations. Freezing rates which produce optimum recovery are all rapid and require liquid nitrogen temperature for storage. The mechanism by which freezing injures living cells has been extensively investigated. A discussion is made of a series of experiments undertaken to determine whether an exchange of extracellular and intracellular electrolytes might be a necessary component of the recovery of red cells following rapid freezing with extracellular cryoprotective agents.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1971
- Accession Number
- AD0746840
Entities
People
- H. T. Meryman
- M. Hornblower