Preservation of Mammalian Tissues and Organs by Freezing.
Abstract
Further support has been obtained for the minimum volume hypothesis of freezing to mammalian cells. When freezing is slow with extracellular ice the injury results primarily from the concentration of extracellular solutes and is apparently related to their osmotic effects. Studies with potassium-depleted human red cells show that injury is unrelated to the absolute osmolality of the suspending solutions or the concentration of intracellular electrolyte. Injury beings to occur with the loss of 65% of the normal cell water content. Spinach chloroplast grana do not reach critical volume and remain uninjured at any concentration or temperature. It is proposed that cells mechanically resist volume change and that in hypertonic suspension they are not in equilibrium with their environment but have an osmotic pressure gradient across the membrane. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 1972
- Accession Number
- AD0746898
Entities
People
- Harold T. Meryman