Nerve Homograft Studies.
Abstract
The principal objectives of this research were to develop methods for (1) the treatment of donor nerve to remove as much of the antigenic components as possible, and (2) for nerve storage. Several methods were studied to facilitate the removal of antigenic material including osmotic shock, digestion with proteolytic enzymes, lipases, phospho-lipases, and related enzymes. Following the removal of the antigenic material from the nerve, the nerves were treated with low concentrations of cross linking reagents to decrease the subsequent destruction of the structural material by enzymes from cells attacking the transplant site. Finally, the extracted nerves were stored in aqueous DMSO or Glycerin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 30, 1971
- Accession Number
- AD0746899
Entities
People
- Franklin L. Ashley
Organizations
- University of California, Los Angeles