The Effect of pH and Inosine on the Regeneration of ATP, 2,3-DPG and P(50) of Blood Incubated at 1C. and 37C.
Abstract
The technical objective of the work reported was to develop practical methods to maintain the highest level of 2,3-DPG and oxygen dissociation along with a reasonable survival. ATP values of 69% plus or minus 2 compatible with 70% survival were achieved with any of the methods reported. Best function, as reflected by 2,3-DPG and P(50) values, was achieved when blood was collected into ACD pH 5.6 and incubated at 37C. with inosine at pH 7.0 or 7.2. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1972
- Accession Number
- AD0750637
Entities
People
- Paul V. Strumia
Organizations
- Bryn Mawr Hospital