Indirect Hemagglutination Test to Detect Antibodies for Tickborne Encephalitis Virus,
Abstract
The possibility of using the indirect hemagglutination test (IHA) with native goose erythrocytes is utilized in the detection of antibodies for tick-borne encephalitis virus. The relationship between the antibody titers in the IHA test and conditions of sensitization and performance of the reaction are studied. Sensitization is achieved by incubating a 2% suspension of erythrocytes in a phosphate buffer (5.8 - 6.0 pH) with an equal volume of antigen for a period of 15 minutes at 37 degrees. The sensitized erythrocytes should be added to the serum solutions in the form of an 0.33% suspension in a dextrose-gelatine-veronal buffer. The reaction may take place at 4 degrees or at room temperature. The IHA is specific and more sensitive than the presently used HI test. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1972
- Accession Number
- AD0754337
Entities
People
- R. D. Margolin
- Z. N. Kondrashov
Organizations
- United States Army Medical Research Institute of Infectious Diseases