Basic Studies on Interactions of Agents Causing Respiratory Infections
Abstract
The importance of respiratory infections, especially the probable interaction of respiratory pathogens, in military populations was the basis of this investigation. It had been demonstrated that M. pneumoniae enhanced rhinovirus 1A/2060-RNA synthesis. Another rhinovirus (16/11757) was studied under similar conditions and also showed enhanced viral-RNA synthesis in M. pneumoniae infected cells. To demonstrate any close relationship between rhinovirus 1A/2060 and M. pneumoniae and M. orale, electron microscopy studies were conducted. These electron micrographs showed no virus attached to mycoplasma or intracellular rhinovirus crystals in the M. pneumoniae or M. orale cells. A study was conducted to determine the effect of these viruses on 3H-thymidine uptake of M. pneumoniae. This investigation showed viable virus as well as heat inactivated virus resulted in decreased 3H-thymidine uptake. Further studies to detect a protective effect of mycoplasma on viruses demonstrated that rhinovirus had a similar survival rate at 37 deg C in the presence or absence of mycoplasma without mammalian cells. Influenza virus was partially inactivated at 60 min exposure to M. pneumoniae, compared to the control without mycoplasma. At 120 mins exposure both systems had a decrease in viral titer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 15, 1973
- Accession Number
- AD0754657
Entities
People
- Ronald D. Fletcher
Organizations
- University of Pittsburgh