A New Technique for Evaluating Antigenic Relatedness Among Viruses
Abstract
The ability of the macrophage migration inhibition (MMI) test for differentiating both bacterial viruses and two cross-reacting strains of the avian coronavirus, infectious bronchitis virus. During the past year, the authors have demonstrated that Adenovirus types 1, 4, 5 and 7 are all clearly separable by MMI test procedures. It was also determined that the optimal sensitizing dose for Adenovirus type 1 was 100 micrograms per pig and that doses above or below this level gave poorer responses. In addition, two nuclear polyhedrosis viruses (NPV) which infect the alfalfa looper and cabbage looper (two important insect pests) respectively were clearly differentiated by MMI test procedures. The polyhedral sizes, shapes and virion occlusion patterns of the two species of NPV were indistinguishable. Attempts to passively transfer the delayed response to normal peritoneal exudate cells by using RNA extracts of tissues from sensitized animals have thus far been unsuccessful.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1973
- Accession Number
- AD0757729
Entities
People
- Charles V. Benton
- David Vic
- Frank M. Hetrick
- James F. Novotny
Organizations
- University of Maryland