A Proposal to Study the Role of the Adrenal Cortex in Maintaining the Androgenic-Anabolic Status in Humans under Normal and Stressful Experimental Conditions.
Abstract
A method was developed for the isolation of urinary testosterone from urinary testosterone glucuronide, followed by purification of the testosterone on an LH-20 column, and quantitation by radioimmunoassay. Reasonable testosterone excretion values, in urine samples from normal volunteers, compatible with published data, were obtained. Methods for the isolation of testosterone, androsteneidione, and 11 beta-hydroxyandrostenedione from plasma have been studied, tested and evaluated. Satisfactory isolations of the steroids of interest were obtained using LH-20 columns. A method for preparing plasma extracts containing the steroids of interest, which were adequate from the quantitative point of view, and could be utilized satisfactorily on LH-20 columns was developed. It is felt that the extraction and isolation procedure is qualitatively and quantitatively satisfactory, and suitable for routine use on multiple columns run simultaneously. (Author Modified Abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1973
- Accession Number
- AD0758262
Entities
People
- Joseph W. Goldzieher
Organizations
- Texas Biomedical Research Institute