Formalin-Inactivated Venezuelan Equine Encephalomyelitis (Trinidad Strain) Vaccine Produced in Rolling-Bottle Cultures of Chicken Embryo Cells,

Abstract

Formalin-inactivated Venezuelan equine encephalomyelitis vaccine was prepared from virus grown in rolling-bottle cultures of chicken embryo cells. Trinidad strain virus was propagated in these cultures with a maintenance medium consisting of serum-free medium 199 containing 0.25% human serum albumin (USP) and antibiotics. Manipulation of multiplicity of inoculum (0.06 to 0.00006) and maintenance medium volume (100 to 300 ml) resulted in high-titered virus yields and only moderate cell destruction when fluids from infected cultures were harvested at 18 to 24 hr. The virus was inactivated at 37 C by 0.05% Formalin within 8 to 10 hr and with 0.1% Formalin within 6 to 8 hr. Single dose, antigen extinction tests in mice performed with 30 small-scale vaccine lots showed excellent potency at either Formalin concentration with inactivation periods ranging from 24 to 96 hr.

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Document Details

Document Type
Technical Report
Publication Date
Oct 27, 1972
Accession Number
AD0759456

Entities

People

  • David M. Robinson
  • Francis E. Cole Jr.
  • Stephen W. May

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Albumins
  • Anti-Bacterial Agents
  • Arbovirus Infections
  • Culture Techniques
  • Encephalitis
  • Equine Encephalitis
  • Health Services
  • Materials
  • Medical Personnel
  • Public Health
  • Rodents
  • Venezuelan Equine Encephalomyelitis
  • Viruses

Fields of Study

  • Biology

Readers

  • Immunology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology