A Multipurpose Automated System for the Simultaneous Assay of Methemoglobin and Total Hemoglobin.

Abstract

A sensitive and accurate automated method for the simultaneous measurement of methemoglobin and total hemoglobin blood levels is described. The method can be used for either continuous in vivo monitoring or intermittent sampling at a rate of 40 per hour. Standardization procedures are described, using both commercial bovine methemoglobin and human blood treated with potassium ferricyanide. Repeatability of both methemoglobin and total hemoglobin measurements expressed as percent of the coefficient of variation is within plus or minus 2 percent. Methemoglobin levels in normal humans and dogs did not exceed 0.55 gram per 100 milliliters (3.5 percent of the total hemoglobin levels). (Author)

Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1973
Accession Number
AD0759912

Entities

People

  • Andris Kaminskis
  • Samuel A. Cucinell
  • William A. Groff Sr.

Tags

DTIC Thesaurus Topics

  • Chemical Compounds
  • Coefficients
  • Control Systems
  • Cyanides
  • Hemoglobin
  • Measurement
  • Methemoglobin
  • Monitoring
  • Multipurpose
  • Potassium
  • Sampling
  • Standardization

Fields of Study

  • Medicine

Readers

  • Analytical Chemistry
  • Cardiovascular Physiology