Fractionation of Toxins from 'Hydrophis cyanocinctus' Venom and Determination of Amino Acid Composition and End Groups of Hydrophitoxin

Abstract

Three toxic fractions were separated from crude venom of the sea snake Hydrophis cyanocinctus by column chromatography. The most abundant fraction, called Hydrophitoxin a, was studied for amino acid composition and found to contain 19 of the common amino acids (including asparagine and glutamine); phenylalanine is absent. The total number of amino acid residues for molecule is estimated to be 59. Methionine occupies the N-terminal position and asparagine is at the C-terminal end. From these preliminary results Hydrophitoxin a appears to be similar in molecular size and structure to several other sea snake venoms previously reported.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1973
Accession Number
AD0760609

Entities

People

  • C. S. Lin
  • C. S. Liu
  • G. S. Huber
  • R. Q. Blackwell

Organizations

  • Naval Medical Research Center

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical Research
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Column Chromatography
  • Hemoglobin
  • Materials
  • Methionine
  • Molecules
  • Navy
  • Peptides
  • Proteins
  • Sodium
  • Sodium Compounds
  • Sodium Hydroxide
  • Thin Layer Chromatography

Fields of Study

  • Biology

Readers

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  • Molecular Genetics
  • Superconducting Magnet Technology