Factors Affecting the Level of Infectivity of RNA of Venezuelan Equine Encephalomyelitis (VEE) Virus

Abstract

RNA of the Venezuelan equine encephalomyelitis (VEE) virus, isolated from virions and infected cells was studied, and the main parameters for detection of maximal infectivity of the viral RNA were determined. It was shown that, among the factors determining the RNA infectivity level, adsorption time of the RNA on cells, the concentration and duration of treatment of the cells with NaCl, and the addition of polycations (DEAE-dextran, protamine sulfate) to the agar overlay, are all of significant importance. The data obtained in the study suggest optimal conditions for determining the infectious activity of the RNA of the VEE virus.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1973
Accession Number
AD0760633

Entities

People

  • A. S. Agabalyan
  • F. I. Ershov
  • L. K. Menshikh

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Adsorption
  • Arbovirus Infections
  • Biomedical Research
  • Cells
  • Culture Techniques
  • Immune Serums
  • Indicator Dyes
  • Infection
  • Infectious Diseases
  • Materials
  • Monomolecular Films
  • Nucleic Acids
  • Ribonuclease
  • Ribonucleic Acids
  • Venezuelan Equine Encephalomyelitis
  • Viruses

Fields of Study

  • Biology

Readers

  • Environmental Engineering
  • Virology (or Medical Virology).