Interferon and an Interferon Antagonist

Abstract

Previous research has found crude Newcastle disease virus (NDV) - induced interferon produced in L-929 cell cultures to be highly effective in protecting these cells against Mengovirus but totally incapable of protecting mouse-passaged Ehrlich ascites tumor (EAT) cells. This anamolous intraspecies specifity was due to the presence in the crude material of an interferon antagonist. Adsorption on CM Sephadex C25 and elution with a rising pH gradient effected isolation of semipurified interferon, fully protective of EAT cells, as well as separation of the antagonist. The latter eluted in two positions - one just after the loading volume and the other just prior to interferon elution. This report further characterizes the antagonist by biochemical and biological parameters.

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Document Details

Document Type
Technical Report
Publication Date
Oct 31, 1970
Accession Number
AD0762072

Entities

People

  • Annie R. Beasley
  • M. M. Sigel

Organizations

  • University of Miami

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Animal Diseases
  • Body Fluids
  • Cell Count
  • Cells
  • Culture Techniques
  • Dilution
  • Health Services
  • Incubation
  • Interferon
  • Materials
  • Membranes
  • Military Research
  • Mixing
  • Mixtures
  • Monomolecular Films
  • Proteins

Fields of Study

  • Biology

Readers

  • Fluid Dynamics.
  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).