A Yeast Cell Sugar Compartment. I. Sorbose Uptake.
Abstract
Sorbose uptake by Saccharomyces cerevisiae was increased 40 to 60% by glucose and other fermentable sugars. Iodoacetic acid prevented the sugar effect, but cycloheximide did not. However, pretreatment of the cells with iodoacetic acid increased uptake. Neither growth nor sorbose binding were adequate to account for the increased uptake. Water space measurements showed that only 65 to 75% of available space was normally occupied by sorbose, and that addition of glucose resulted in more complete filling. Dimethylsulfoxide, which enters yeast by simple diffusion, largely filled the available water space. It is concluded that a restrictive sugar compartment prevents or slows maximum entry of sorbose into these cells; metabolizable sugars and iodoacetic acid open the compartment. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 23, 1973
- Accession Number
- AD0765411
Entities
People
- Edward Spoerl
- J. P. Zahand
- S. N. Lowery
Organizations
- United States Army Medical Research Laboratory