Microbial Drug Resistance (Epidemiology, Genetics).
Abstract
Antibacterial activity of aminoglycosides toward Pseudomonas aeruginosa was investigated by using 221 clinical isolates. One hundred and seventy-five strains were shown to be highly resistant against one or more of the antibiotics tested. A lividomycin-phosphorylating enzyme from a lividomycin-resistant strain of Escherichia coli carrying an R factor was partially purified by fractionation with ammonium sulfate and Sephadex G-100 column chromatography. The enzyme inactivated, several antibiotics having a D-ribose moiety linked to 2-deoxystreptamine, i.e., lividomycin A and B, neomycin, paromomycin, and vistamycin, but did not inactivate the kanamycins, streptomycin, or the gentamicin C components. The gentamicin acetylating enzyme from a resistant strain of Pseudomonas aeruginosa was purified by ammonium sulfate fractionation and column chromatography. The enzyme inactivated gentamicin C1, C2, and Cla in the presence of acetylcoenzyme A but did not inactivate other aminoglycosidic antibiotics such as paromomycin, mannosylparomomycin, dihydrostreptomycin, neomycin, kanamycins. The dihydrostreptomycin was inactivated by adenylylation of the drug in the prescence of both ATP and Mg(2+) by carrying an intermediate SM-resistant strains of S.aureus. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 20, 1973
- Accession Number
- AD0768735
Entities
People
- Susumu Mitsuhashi