Studies of the Mammalian Brain Function in vitro.
Abstract
Effects of long term (7 1/4 and 5 3/4 years) storage of the cat in frozen state at -20C in vitro after employing a glycerol perfusion technique were investigated from modes of neuroelectrical activity and histological evidences. The following conclusions were obtained: Glycerol, even if administered via vascular systems, showed cryoprotective effects on the brain tissues as was the case of single cells; it was possible to preserve viability of nervous tissues against many years storage in frozen state; reperfusion for revival brought about enlargement of micro-clefts in tissues which might have been produced by thawing of long term frozen tissues. This may be a cause of bleeding and activity defect in the revived brain; integrated neuroelectrical activity such as brain waves and evoked potentials may be affected significantly by dissolution of functions resulted from disruption in tissues; individual nerve cell activity, on the contrary, may be preserved well essentially or augmented in activity by degenerative processes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1972
- Accession Number
- AD0768737
Entities
People
- Isamu Suda
Organizations
- Kobe University