The Integration of Cellular and Humoral Responses to Protozoan Parasites.
Abstract
The immunological mechanisms against Plasmodium berghei and Leishmania tropica have been studied. The humoral component of anti-malarial immunity was investigated by neutralization of small infective inocula. The neutralizing antibody in hyperimmune rat serum is in the IgG fraction. Optimal amounts of the serum neutralize, while suboptimal doses may enhance, the infection. Neutralization occurs in vivo in the recipient rat. Splenocytes from malarious rats adoptively transfer immunity up to the third passage. Thymidine incorporation by such splenocytes is enhanced for the first eight hours in culture. The serological and physiological properties of a factor (EF) excreted by leishmania promastigotes in vitro have been described and used as a basis for leishmanial serotyping. Phagocytic activity of leishmania infected macrophages is suppressed while lysosomes and lysosomal enzyme production are enhanced. The lysosomal membranes are stabilized, possibly by EF excreted by the amastigotes, preventing discharge of the lysosome into the phagosome. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1973
- Accession Number
- AD0769946
Entities
People
- Avivah Zuckerman
- Charles L. Greenblatt
- Dan T. Spira