Activity of Some Glycolysis Enzymes in the Liquid Phase of Tissue Cultures Infected with Venezuelan Equine Encephalomyelitis Virus,
Abstract
The reproduction of Venezuelan equine encephalitis virus in cultures of chick and human embryo fibroblasts is accompanied by changes in the activity of some enzymes of carbohydrate metabolism (glucosophosphatisomerase, aldolase, and lactatedehydrogenase). The enzymatic changes in the cells were determined by the changes in enzymatic activity in the tissue culture fluids. The experiments performed demonstrated that the activity of glucosophosphatisomerase increases so early as 4 hours after infection of fibroblasts, before cytopathic changes develop in the cells. Lactatedehydrogenase activity also exhibited a statistically significant increase before the appearance of cytopathic changes. Unlike the activity of these two enzymes, the aldolase activity of the medium did not change in advance of cell sheet degeneration and did not increase until after the death of the cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1972
- Accession Number
- AD0770294
Entities
People
- E. A. Peshkova
- T. L. Miryutova
Organizations
- United States Army Medical Research Institute of Infectious Diseases