Yeast Phenylalanyl Transfer Ribonucleic Acid Synthetase: Purification and Complex Formation with Phenylalanine Transfer Ribonucleic Acid.
Abstract
It has been found that a homogeneous preparation of phenylalanyl aminoacyl- tRNA synthetase (phe-synthetase) from yeast can be obtained if phenylmethylsulfonyl fluoride is used in all buffers and if phosphocellulose chromatography is employed as a terminal step in the purification. Molecular weight was determined, and complexes between phe-synthetase and tRNA(Phe) were observed in the analytical ultracentrifuge. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1973
- Accession Number
- AD0770376
Entities
People
- Karl W. Lanks
- Liama Baltusis
- Robert K. Eng