Study of the Protein Components of Venezuelan Equine Encephalomyelitis with Use of Electrophoresis and Isoelectric Focussing

Abstract

The presence of three protein components with molecular weights of 59,000, 34,000 and 18,000 daltons was determined from mobility in polyacrylamide gel containing sodium dodecysulfate. The method of isoelectric focussing in two modifications (column focussing and focussing on the basis of disk polyacrylamide gel) was used to demonstrate also the presence of three protein- containing components in low-acid, neutral and low alkaline zones of pH gradient both by ultraviolet adsorption and by radioactive label.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 1973
Accession Number
AD0772873

Entities

People

  • L. V. Uryvaev.
  • V. M. Zhdanov
  • Yu. S. Derkich

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetic Acid
  • Algae
  • Arbovirus Infections
  • Arboviruses
  • Biomedical Research
  • Cells
  • Culture Techniques
  • Electrodes
  • Electron Microscopes
  • Electrophoresis
  • Filter Paper
  • Materials
  • Molecular Weight
  • Proteins
  • Venezuelan Equine Encephalomyelitis
  • Viral Structures
  • Viruses

Readers

  • Molecular and Cellular Biochemistry
  • Spectroscopy.