The Obtaining of Bacterial Allergens by the Alkaline-Extraction Method and the Investigating of their Physico-Chemical Properties

Abstract

A common method of obtaining allergens from bacteria of the group of particularly dangerous infections and of certain representatives of the enteric family is suggested. The method consists in the extraction from microbial cells of alkali-soluble components, their precipitation with acid, dialysis and lyophilization. The protein part of the allergens mainly contained the fraction with a high molecular weight. Thermal treatment of certain allergens (tularemic and typhoid) was accompanied by the rupture of the bond in the protein molecule, and the separation of fractions with a lesser molecular weight; some retained their specific activity.

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Document Details

Document Type
Technical Report
Publication Date
Jan 07, 1974
Accession Number
AD0773521

Entities

People

  • V. F. Runova
  • V. Yu. Gavrilenkova

Organizations

  • National Air and Space Intelligence Center

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Animals
  • Bacteria
  • Chemical Properties
  • Chemistry
  • Escherichia
  • Escherichia Coli
  • Filtration
  • Foreign Technology
  • Gram-Negative Bacterial Infections
  • Infection
  • Microorganisms
  • Molecular Weight
  • Nucleic Acids
  • Rodents
  • Vaccines
  • Wound Infections

Readers

  • Allergy and Immunology.
  • Analytical Chemistry
  • Immunology

Technology Areas

  • Biotechnology