Serological Grouping of Hemolytic Streptococci by Counter- Immunoelectrophoresis

Abstract

The counter-immunoelectrophoresis (CIE) method of grouping streptococci was more sensitive than the capillary precipitin method. The precipitate was easier to read, and the test was simple to perform and required fewer reagents. An autoclave-Pronase-B extraction procedure is described and was found superior to either acid, Pronase-B, or the autoclave extraction procedures for recovering groupable polysaccharide from different strains of streptococcal groups A, B, C, D, F, and G. Data obtained from over 400 strains indicate that the sensitivity of the CIE and a more efficient extraction of groupable polysaccharide provides a simple method for rapid diagnosis of streptococcal infections.

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Document Details

Document Type
Technical Report
Publication Date
Jul 09, 1973
Accession Number
AD0776458

Entities

People

  • Earl A. Edwards
  • George L. Larson

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Autoclaves
  • Biomedical Research
  • Blood
  • Capillary Tubes
  • Carbohydrates
  • Extraction
  • Great Lakes
  • Health Services
  • Immune Serums
  • Infection
  • Microvessels
  • Polysaccharides
  • Precipitates
  • Security
  • Sensitivity
  • Streptococcus

Readers

  • Analytical Chemistry
  • Immunology