Sensitivity to Hemolysis of Canine and Human Red Cells after Timed Exposure to Solutions of Varying pH.
Abstract
Subjecting human and dog red cells to the stress of nonphysiological pH was found to lead to rapid hemolysis in the dog cells without a significant effect on the human cells. A variety of incubation temperatures and times was used with relatively little difference and with all canine specimens showing hemolysis by 24 hours. Use of solutions with varying pH demonstrated quick onset of hemolysis in alkaline solutions, and delayed lysis in markedly acid solutions. An overall tendency for the pH to rise regardless of the initial starting level, was observed. Upon reaching the alkaline range, lysis of red cells usually occurred. Lysis in the very low acid range could occur earlier and while still in an acid pH range. The narrowness of the range of pH tolerated by canine red cells was in contrast to the broad range tolerated by human erythrocytes. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 21, 1973
- Accession Number
- AD0778720
Entities
People
- Angelo I. Zegna
- Charles E. Shields
Organizations
- United States Army Medical Research Laboratory