Alterations in Oxygen Transport Observed in Stored Blood Drawn from Normal or Chronically Anemic Dogs.
Abstract
Units of blood were drawn from normal and anemic dogs and stored in acid citrate dextrose for six weeks. The units were sampled at intervals and tested for oxygen dissociation P50 point, pH, acid citrate dextrose, 2,3 diphosphoglycerate levels, and methylene blue uptake, as well as osmotic fragility. The general loss of these with storage paralleled the findings obtained with normal bank blood. One particular exception was the finding of higher 2,3 diphosphoglycerate levels in anemic blood that tended to be higher than normal throughout storage. Anemic cells were also found to be more resistant to osmotic lysis, possibly because of their microcytic shape. The biological response to the degree of chronic anemia induced in these dogs appeared to be primarily an increase in 2,3 diphosphoglycerate level without marked alteration in the oxygen curve or other investigated metabolic factors. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 27, 1973
- Accession Number
- AD0778722
Entities
People
- C. E. Shields
- D. E. Brooks
- D. E. Meixner
- F. Devenuto
- J. F. Bratton
Organizations
- United States Army Medical Research Laboratory