Some Effects of DBcAMP on Rabbit Bone Marrow Cells in Culture: Cell Clumping and Cholinesterase Production.
Abstract
Bone marrow cells derived from phenylhydrazine-treated rabbits were grown in vitro for 24 hours. Marrow cultivated in the presence of .001 M dibutyryl 3',5'-cyclic monophosphate (DBcAMP) formed large, dense cell clusters whereas those from control cultures formed smaller, more discrete cell clusters. Actinomycin D inhibited cluster formation. Erythroid cell maturation was also inhibited in the presence of DBcAMP. However, cholinesterase synthesis was enhanced. Most of the cells containing newly synthesized membrane-bound cholinesterase are erythroid. On the basis of the data obtained, it is suggested that the cyclic nucleotide may stimulate membrane protein synthesis. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1974
- Accession Number
- AD0780009
Entities
People
- John P. Petrali
- Larrel W. Harris
- Vincent F. Garry Jr.