Cultivation of Hepatitis Virus in Tissue Culture.
Abstract
A carefully planned program with the eventual goal, being the development of a live viral vaccine for hepatitis, was undertaken at the UCLA Center for the Health Sciences. The initial phase of this program aimed at the development of tissue culture substrates for use in vaccines. Reproducible and reliable techniques have been developed for the short and long term maintenance of hepatocytes in monolayer tissue culture. Using colagenase in association with an argenine deficient selective medium, fetal and adult hepatocytes have been maintained in fibroblast free cultures for periods of up to 5 months. On subculture, primary cultures maintain their epithelial characteristics. Hepatitis B Antigen has been maintained intracellularly in these cultures for periods of up to 12 days before titers have fallen off. Evidence of replication exists in that these periods, in cell free media, titers fall off at a much more rapid rate. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1974
- Accession Number
- AD0782733
Entities
People
- G. L. Gitnick
Organizations
- University of California, Los Angeles