Mechanisms of Hemolysis.
Abstract
Erythrocyte damage by surface damage, shear stress or osmotic lysis was accompanied by the release of free glyceric acid/bis(dihydrogen-phosphate) (DPG) to the plasma in greater concentrations than normally exist within the cell, compared to hemoglobin. Following incubation, DPG was rapidly depleted in both control and damaged specimens, and the oxyhemoglobin dissociation curve was shifted to the left of an appropriate degree. Red blood cell adenosine triphosphate (ATP) concentration was substantially depleted only after osmotic lysis, although free plasma ATP was demonstrable following both shear and osmotically induced injury. Platelet adhesiveness is markedly decreased by surface injury. Platelet aggregation in response to adenosine phosphates, collagen and thrombin is markedly reduced following surface injury. Platelet Factor 3 availability is decreased following surface injury, but not following shear stress, although Platelet Factor 3 content is not significantly altered after either form of damage. (Modified author abstract)
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1974
- Accession Number
- AD0784280
Entities
People
- Eugene F. Bernstein
Organizations
- University of California, San Diego