Determination of Subpicogram Quantities of Drugs and Narcotics in Urine of Human Subjects.
Abstract
A feasibility study of the use of field ionization mass spectrometry for the analysis of drugs and narcotics in urine by isotope dilution was completed. Multilabeled methaqualone, 6-hydroxymethaqualone, morphine, and codeine were synthesized. The drugs and their isotopic carriers were extracted from urine on columns of Amberlite XAD-2 resin and were purified by one-dimensional thin-layer chromatography. The isotopic compositions of the extracted compounds were determined by mass spectrometry. Analyses of the methaqualone and 6-hydroxymethaqualone concentrations in a few urine samples obtained from human volunteers indicate that the sensitivity of the present technique is of the order of nanograms/ml. More effective purification steps and the use of higher purity carrier compounds will permit the analysis of lower drug concentrations. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1974
- Accession Number
- AD0784632
Entities
People
- Henry D'a. Heck
- James H. Mcreynolds
- John A. Lawson
- Michael Anbar
Organizations
- SRI International