A Modified Histochemical Procedure for Measuring Succinic Dehydrogenase (SDH) in Corneal Epithelium.
Abstract
Succinic dehydrogenase (SDH), which is critical to maintaining the transparency of the cornea, is utilized in the aerobic metabolism of glucose via the tricarboxylic acid (Krebs) cycle. What happens to this enzyme when the cornea is deprived of normal concentrations of oxygen (when contact lenses, masks, etc., are worn) is not known. According to previous studies, SDH was either present in corneal epithelium in barely detectable amounts or the techniques used for measurement were not sensitive enough to detect all the SDH present. As a part of the study, the authors modified existing techniques of detecting SDH in corneal epithelium. The intensity of staining SDH was enhanced by modifying a medium containing tetrazolium to the point where it was sensitive enough for quantitative photometric measurements. The existing techniques were modified by adding a coenzyme, beta-diphosphopyridine nucleotide (DPN), and increasing the amounts of specific substrate, sodium succinate. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1974
- Accession Number
- AD0785526
Entities
People
- John P. Petrali
- Kenneth R. Mills
- Roy H. Rengstorff