ELIMINATION OF PLEUROPNEUMONIA-LIKE ORGANISMS FROM TISSUE CULTURE

Abstract

Tissue cultures heavily contaminated with PPLO were treated with kanamycin (1 mg/ml of tissue culture medium for 48 hours), kanamycin in combination with heat (41 C for 18 hours) or novobiocin (50 mg/ml of tissue culture medium for 7 days) in combination with heat. Only treatment with novobiocin and heat was successful in ridding the cultures of the PPLO contamination.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1966
Accession Number
AD0800591

Entities

People

  • Michael D. Orlando
  • Roger W. Johnson

Tags

Communities of Interest

  • Cyber
  • Materials and Manufacturing Processes

DTIC Thesaurus Topics

  • Contamination
  • Culture Media
  • Culture Techniques
  • Elimination
  • Glycosides
  • Heat Energy
  • Heat Treatment
  • Life Cycles
  • Literature
  • Maryland
  • Tissue Culture
  • United States Military Academy

Fields of Study

  • Agricultural and Food sciences

Readers

  • Microbial Pathology
  • Trauma Surgery or Emergency Medicine.