STUDIES OF BIOLOGICALLY ACTIVE AGENTS IN CELL AND TISSUE CULTURES. PART 1
Abstract
Toxins Produced by Microrganisms - The cytotoxicity of Staphylococcal enterotoxin B to cells of human embryonic intestine is markedly reduced by trypsin. The temporary resistance increases proportionally with increased time of exposure to trypsin and lasts for 48 hours. It appears that trypsin inactivates specific cell receptors which may be essential for interaction with enterotoxin. Selected Toxic Substances - Organochlorine and organophosphorus compounds (inhibitors of acetylcholinesterases) including DDT, chlordane, and malathion at subtoxic concentrations inhibit vaccinia virus replication in human Chang liver cells. The replication of poliovirus is inhibited only by chlordane and malathion. Nutritional Factors in Viral Infections - A deficiency of methionine and glycine induced by their analogues, and a deficiency of leucine in the medium suppress vaccinia virus replication significantly more than they inhibit the growth of cells. The replication of virus is also inhibited when the medium contains an excess of either methionine, glycine, leucine, isoleucine, or valine. The inhibitory effect of ethionine on virus replication in cell cultures correlates well with the results obtained with vaccinia virus infection in the rabbit.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1966
- Accession Number
- AD0804387
Entities
People
- Janis Gabliks
Organizations
- Massachusetts Institute of Technology