STUDIES ON PROTEOLYTIC, FIBRINOLYTIC, ANTITRYPTIC AND ANTIFIBRINOLYTIC ACTIVITIES IN THE BLOOD OF RATS,
Abstract
In order to elucidate further the sequences of increased protein catabolism observed under various conditions of stress, the following experiments were carried out: The proteolytic and antitryptic activity was determined in the blood of rats during 'tourniquet shock.' The proteolytic activity of plasmin was compared with that of crystalline trypsin, using casein and fibrinogen as substrates. The fibrinolytic and antifibrinolytic activity was determined in plasma of rats in 'tourniquet shock' and after epinephrine administration to normal rats. No change in proteolytic activity could be demonstrated in the serum of rats in 'tourniquet shock,' using the degree of liberation of tyrosine-tryptophane from a casein substrate as an index of proteolytic activity. The antitryptic titer in the same animals, measured on crystalline trypsin with casein as a substrate, was slightly lowered, compared to normal. Comparison of the fibrinogenolytic and proteolytic activities of trypsin, plasmin and chloroform activated serum enzyme indicated parallelism between these enzymes. This similarity further substantiates the claim that plasmin can act as a proteolytic enzyme and may well play a significant role in the process of protein breakdown.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 30, 1950
- Accession Number
- AD0806418
Entities
People
- Bertram K. Mcdonald
- Elma W. King
- Hans F. Jensen
- Ulrich F. Westphal
- Walter Kocholaty
Organizations
- United States Army Medical Research Laboratory