A COMPARISON OF VARIOUS LIPID EXTRACTION METHODS AND THEIR APPLICATION TO THE DETERMINATION OF TOTAL LIPIDS IN ESCHERICHIA COLI, STRAINS B AND B/R, WHEN GROWN UNDER VARIED ENVIRONMENTAL CONDITIONS,
Abstract
The purpose of this inquiry was to apply a highly specific microextraction procedure to the determination of the total lipid content of Escherichia coli, strains B and B/r. Several lipid extraction procedures were compared and evaluated. Employing the microextraction method of Hsiao (11) for the determination of total lipids in Excherichia coli, it was found that the lipid contents of E. coli B and B/r, when grown on a mineral medium with acetate as the only carbon source, did not differ significantly. The total lipid content of E. coli showed significant differences depending upon the environmental conditions to which the cells were exposed. A decrease in the total lipid content was observed during the spontaneous dark recovery phase of ultraviolet irradiated cells of E. coli B. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 14, 1958
- Accession Number
- AD0806444
Entities
People
- E. E. Woodside
- W. Kocholaty
Organizations
- United States Army Medical Research Laboratory