SYNCHRONIZED BUDDING OF YEAST CELLS FOLLOWING X-IRRADIATION,
Abstract
Yeast cells, following a continuous 3 hour treatment with x-radiation which inhibited cell division but not growth, resumed budding in a synchronous fashion. Though budding was resumed, cell separation did not occur, and consequently, groups of four cells resulted from budding of the paired cells typical of the stage of inhibited division. A second budding produced still larger cell groups. Measurements of the uptake of radioactive phosphorus by divison-inhibited cells and by cultures with 80% of the cells ready to bud showed a greater uptake per unit of dry weight by the inhibited cells. The total phosphorus and the phosphorus content of various separated fractions were higher in inhibited cells than in cells ready to divide. Insoluble polyphosphates of inhibited cells showed an accelerated uptake, while soluble polyphosphates took up radioactive phosphorus at a slower rate. Nucleic acids showed no reduced uptake. These phosphorus differences, including the possible specific involvement of polyphosphates in division processes, are considered as leads in uncovering the specific reactions affected by radiation in the processes of cell division. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 22, 1958
- Accession Number
- AD0806446
Entities
People
- Della Looney
- Edward Spoerl
Organizations
- United States Army Medical Research Laboratory