STUDIES ON THE COCCIDIUM, EIMERIA TENELLA,
Abstract
Essentially pure sporulated Eimeria tenella oocyst suspensions, suitable for experimental purposes, were obtained by shaking at 30 C homogenized infected chicken ceca in 2.5% potassium dichromate, digesting extraneous material with 3% KOH at 40 C (in the presence of fecal debris, with an additional incubation in 70% H2SO4), and further purifying by selective recovery from the aqueous phase of a chloroform-water suspension. A quantitative assay of infecting dose in chicks could be made by harvesting ceca (after 7 days) and counting total oocysts; oocyst viability could be determined by per cent in vitro excystation of sporocysts. Oocysts failed to survive freezing in liquid nitrogen or drying, but survived for short periods at -5 to -30 C. Ammonium hydroxide, aqueous iodine, and ethylene oxide were the only disinfectants found effective against E. tenella oocysts. Cross-infection studies with other animal species were uniformly unsuccessful. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1966
- Accession Number
- AD0806775
Entities
People
- Ernest M. Snyder
- John L. Converse
- John P. Kondig