FALSE PRECIPITIN BANDS IN OUCHTERLONY GEL DIFFUSION TESTS DUE TO FILTERABLE BACTERIAL FORMS
Abstract
Bands resembling typical antigen-antibody precipitates developed in Ouchterlony gel diffusion tests between sera from certain individual animals and bacteriologically filtered cultures of Aeromonas salmonicida or Vibrio parahemolyticus. After incubation for several days these bands became coarsely granular and progressively more diffuse. Microsocopic examination revealed them to be made up of massive numbers of what appeared to be microcolonies of barely visible filamentous bacillary forms. All strains of A. salmonicida and V. parahemolyticus tested produced these filterable forms, which were thermolabile but resistant to a number of other physical and chemical agents. Growth of the filterable forms required a particular concentration of serum and medium, which has so far been achieved only by using the Ouchterlony gel diffusion technique. Reversion of these forms to the typical bacillary form has not been observed. No such forms have been detected in cultures of a variety of other fish and human pathogens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1967
- Accession Number
- AD0808908
Entities
People
- Michael J. Surgalla
- Werner A. Janssen