STUDIES ON THE LIMITED GROWTH OF TEMPERATURE-SENSITIVE VEE VIRUS AT 44 C
Abstract
By using a special sampling method, a significant, but limited, increase in Venezuelan equine encephalitis (VEE) virus titer can be detected during early 44 C incubation of VEE-infected chick embryo (CE) cells. The maximum titer is 0.1 to 1% that of cultures incubated at 37C. The magnitude of the increase in titer is not a function of size of virus inoculum. The limitation of the increase was not due to lack of adsorption or to medium alteration at 44C. The best conditions for demonstrating the phenomenon were determined. Effective removal of excess inoculum and medium replacement at 2- hour intervals were optimal for demonstrating early 44 C growth of VEE in CE monolayers. Experiments with metabolic inhibitors and other selective conditions suggest that the increase is due to true de novo viral synthesis and not to elution or recoating of the input viral genome. The limited virus growth at 44 C is especially sensitive to low levels of puromycin compared with growth at 37 C. Possible mechanisms involved are discussed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1967
- Accession Number
- AD0808909
Entities
People
- Eugene Zebovitz
- Sidney Halle