CHROMOSOMAL MAPPING IN STRAINS OF STAPHYLOCOCCUS AUREUS,

Abstract

The nitrosoguanidine method of mapping the chromosome of Staphylococcus aureus is described. The method employs phenylethyl alcohol inhibition of chromosomal replication followed by periodic exposure to nitrosoguanidine after release from the phenylethyl alcohol block. Chromosomal replication requires approximately 60 minutes under the standard conditions employed. A genomic map of a phage type 80/81 strain containing the locations of 10 genes has been obtained. Comparison of a number of isolates of S. aureus from clinical materials for the location of resistance loci to chloramphenicol, novobiocin, erythromycin, nitrofurazone, and acriflavin has shown clear transpositions of some loci. No evidence has been obtained to indicate either a possible circular chromosome or differences in polarity of chromosomal replication among the strains examined. It appears that the order of the resistance genes examined may vary considerably among the strains so far evaluated. (Author)

Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1967
Accession Number
AD0810285

Entities

People

  • Robert A. Altenbern

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Bacteria
  • Chromosomes
  • Glycosides
  • Inhibition
  • Intact Stability
  • Materials
  • Nitroso Compounds
  • Polarity
  • Resistance
  • Standards
  • Staphylococcus Aureus

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Military/Explosive Ordnance Disposal (EOD) Technology
  • Molecular Genetics