EFFECT OF INPUT MULTIPLICITY AND TISSUE CELL CONCENTRATION UPON THE GROWTH OF RIFT VALLEY FEVER VIRUS

Abstract

The growth of Rift Valley fever (RVF) virus in L (Earle) cells obtained from two sources and the effect of input multiplicity and tissue cell concentration upon the growth of the virus are described. The titers obtained in the suspension cultures with the cells from two laboratories were significantly different. With both monolayer culture system and suspension culture system, an input multiplicity of 2.5 resulted in the greatest proliferation of virus. Optimal viral yield was obtained in suspension cultures with 40,000,000 tissue cells per 100 ml of suspension.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1967
Accession Number
AD0810492

Entities

People

  • Jean M. Riley
  • Michael D. Orlando
  • Richard D. Delauter

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Laboratories
  • Blood
  • Cell Line
  • Cells
  • Diseases And Disorders
  • Infection
  • Maryland
  • Microorganisms
  • Monomolecular Films
  • Nutrition
  • Rift Valley Fever
  • Rift Valleys
  • Tissue Culture
  • Valleys
  • Virus Diseases
  • Viruses
  • Wound Infections

Readers

  • Virology (or Medical Virology).