THE HISTOCHEMICAL DEMONSTRATION OF INTESTINAL BETA-D-FUCOSIDASE WITH 5-BROMO-4-CHLOROINDOLE-3-YL-BETA-D-FUCOPYRANOSIDE

Abstract

A halogen-substituted indolyl substrate for beta-D-fucosidase shows strong positive reactions in the interstitial mononuclear cells in the jejunum. This localization and the optimum pH are different from those of the closely analogous substrate for beta-D-galactosidase. The results of competitive inhibition of these substrates suggest that beta-D-fucosidase and beta-D- galactosidase are distinct but similar tissue enzymes.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1967
Accession Number
AD0811014

Entities

People

  • Alfred C. Standen
  • Bjarne Pearson
  • John R. Esterly

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Abstracts
  • Animals
  • Biological Staining And Labeling
  • Cells
  • Chemical Reactions
  • Demonstrations
  • Enzymes
  • Glycosides
  • Inhibition
  • Laboratory Animals
  • Maryland
  • Mucous Membrane
  • Rodents
  • Substrates
  • Three Dimensional
  • Tissues
  • United States

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry