MICRODIFFUSION IODINATION OF PURIFIED MICROBIAL ANTIGENS FOR COPRECIPITATION SEROLOGY

Abstract

Conventional methods for introduction of I131 may modify or destroy the serological reactivity of labile microbial antigens. A modified microdiffusion iodination method that eliminates the exposure of antigens to excess oxidizing agent produced satisfactory trace labeling of purified protective antigen of bacillus anthracis and enterotoxin B of Staphylococcus aureus without detectable change in precipitating activity. The iodinated antigens proved suitable for use in the ammonium sulfate coprecipitation technique of Farr.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1967
Accession Number
AD0819932

Entities

People

  • George G. Wright
  • Jack Gruber

Tags

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Antibodies
  • Biological Toxins
  • Biomedical And Dental Materials
  • Chemistry
  • Immune Serums
  • Laboratory Animals
  • Maryland
  • Materials
  • Molecules
  • Polymeric Films
  • Proteins
  • Proteomics
  • Serology
  • Staphylococcus Aureus
  • United States

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Microbial Pathology
  • Organic Chemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Bioremediation