MULTIPLICATION OF YELLOW FEVER VIRUS IN INSECT TISSUE CELL CULTURES
Abstract
Yellow fever virus (Asibi strain) has been grown successfully in insect tissue culture cell lines from the moth Antheraea eucalypti and the mosquito Aedes aegypti. Increases in virus titer (MICLD50) of two to three logs were evident in 24 to 72 hours at 26 C in a hemolymph-free medium. Virus- infected A. eucalypti cells remained healthy and in a state of reproduction throughout 12 weeks incubation, but in A. aegypti cultures, cell lysis reduced the numbers to 10% of the original count within 120 hours.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1967
- Accession Number
- AD0820847
Entities
People
- John L. Converse
- Stanley C. Nagle Jr.