IDENTIFICATION OF CLONES OF CELLS IN CULTURE BY DISC ELECTROPHORESIS

Abstract

Isoenzyme distribution patterns (zymograms) were obtained from various subclones of an L-Ma clone. On the basis of these zymograms, the various subclones were identified and classified. Preliminary experiments indicate that, although they can be modified by a change in the medium, the zymograms are reliable and useful criteria for identifying clones of cells in culture, when obtained from cells grown under identical and carefully controlled growth conditions.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1967
Accession Number
AD0821459

Entities

People

  • Donald C. Fish
  • James P. Dobbs
  • Jerry S. Walker
  • Richard C. Carter

Tags

DTIC Thesaurus Topics

  • Acids
  • Anatomy
  • Anti-Bacterial Agents
  • Bacteria
  • Biological Sciences
  • Carbon Dioxide
  • Cell Line
  • Cells
  • Cells (Biology)
  • Cultured Cells
  • Enzymes
  • Gel Electrophoresis
  • Identification
  • Isoenzymes
  • Malic Acid
  • United States
  • United States Government

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Regression Analysis.
  • Virology (or Medical Virology).