AN IMPROVED METHOD FOR PARTIAL PURIFICATION OF THE ORGANISM OF PSITTACOSIS GROWN IN CHICKEN EMBRYO YOLK SAC

Abstract

A simple procedure is described for obtaining the organism of psittacosis employing successive extractions with (1) calcium chloride, sodium dextran sulfate, and sucrose (CC-DS-S) and (2) Freon-114. Compared with the CC- DS-S or Freon-114 methods alone, the modified procedure yields a product with only trace amounts of total solids, lipids, and proteins; with increased specific infectivity; and with no loss in biological activity.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 1967
Accession Number
AD0821460

Entities

People

  • Warren G. Dorsey
  • William C. Patrick Iii.
  • William N. Shirey

Tags

DTIC Thesaurus Topics

  • Animals
  • Calcium
  • Calcium Compounds
  • Centrifuges
  • Chlorides
  • Continents
  • Extraction
  • Geographic Regions
  • Gram-Negative Bacterial Infections
  • Laboratory Animals
  • Laboratory Equipment
  • Maryland
  • Materials
  • North America
  • Product Development
  • United States

Readers

  • Analytical Chemistry
  • Neurodegenerative Parkinson's Disease and Rickettsial Disease handbook, including the data level of dopamine, BC, neurons, and PD.
  • Virology (or Medical Virology).