A STUDY OF 'IMMEDIATE' SENSITIZATION BY ADSORPTION OF ANTIGENS AND ANTIBODIES IN VITRO
Abstract
Tetanus: The non-spasmogenic factor in tetanus toxin produces a stimulation of miniature end plate potentials in skeletal muscles. Chromatography on Sephadex G-200 gave 3 major peaks.. No appreciable MEPP activity was found in the first 2 peaks but appeared to be concentrated in Peak III, which had no lethal activity. All of the lethal toxicity of the parent was quantitatively recovered in Peaks I and II which contained 5S and 4S molecules, respectively. The MEPP material accounted for only 7% of the total protein of the starting material. Streptolysin: Streptolysin O gives a double insult to the heart, one of these is reversible, and is due largely to the output of acetylcholine, while the other appears to be more severe and leads to the release of K42. Sea Urchin Toxin: The crude pedicellarial toxin of Tripneustes gratilla obtained from 1,000 specimens in 1966 and 2,000 specimens during 1967 was variously fractionated, and the fractions further purified by chromatography on Sephadex. The enzymological properties of these materials were studied. Ragweed: Rabbit anti-ragweed conferred passive sensitivity on cardiac and intestinal tissues of the guinea pig. The degree of sensitization was shown to depend on the (AB) in the bulk phase. For a constant degree of sensitization the response to the whole antigen was quantitatively inhibited by a dialyzable ragweed hapten. Penicillin: Positive anaphylatic reactions were obtained to penicilloyl poly-lysine in isolated guinea pig tissues passively sensitized with rabbit antibodies. Fc Fragments: Quantitative reversed passive sensitization with Fc fragments of rabbit gamma-globulin was demonstrated.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1967
- Accession Number
- AD0824276
Entities
People
- George A. Feigen
Organizations
- Stanford University