AUTOMATION OF A TECHNIQUE FOR DETERMINING BACTERIAL SENSITIVITY TO ANTIBIOTICS.
Abstract
Development of an automated antibiotic sensitivity test breadboard is proceeding. A partially activated system has demonstrated mechanical operability and the generation of valid data for growth and inhibition in known biological systems. Families of growth curves have been developed for E. coli/penicillin, E. coli/cephalothin and S. faecalis/cephalothin. The shapes of curves and inhibitory effects of antibiotics are similar to those derived earlier with the Lindberg-Reese research instrument. The logic circuitry has been checked and cleared of spurious signals as a preliminary to complete system integration. An empirical method was derived for predicting 24-hour minimum inhibitory concentrations (MIC's), of antibiotics from data taken in the first few hours. It appears capable of predicting MIC's with an uncertainty of one (two-fold) dilution level. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0826331
Entities
People
- G. Reese
- H. H. Anderson
- L. T. Carleton
Organizations
- Aerojet Rocketdyne Holdings