A RAPID TURBIDIMETRIC PROCEDURE FOR COMPLEMENT ESTIMATION

Abstract

Complement (C')-mediated hemolysis of sheep erythrocytes followed a first-order kinetics reaction when measured turbidimetrically. A linear standard curve can be constructed from the slopes of the first-order plots obtained with samples of known C' concentration. The standard curve can then be used for the estimation of C' concentration in unknown sera. This direct kinetic procedure is rapid and accurate and employs a minimum of equipment and reagents.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 1967
Accession Number
AD0827094

Entities

People

  • Earl W. Harris
  • Sidney Yaverbaum
  • William F. Vincent

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Biological Sciences
  • Blood Cells
  • Cells
  • Cells (Biology)
  • Dilution
  • Equations
  • Erythrocytes
  • Hemolysis
  • Kinetics
  • Maryland
  • Materials
  • Physical Properties
  • Sensitivity
  • Standards
  • United States
  • United States Government

Readers

  • Analytical Chemistry
  • Mathematical Modeling and Probability Theory.
  • Regression Analysis.