PATTERN OF VIRAL RNA SYNTHESIS IN A TEMPERATURE-SENSITIVE MUTANT OF EASTERN EQUINE ENCEPHALITIS VIRUS
Abstract
The pattern of viral RNA synthesis of a temperature-sensitive mutant (Ets-4) of eastern equine encephalitis virus was compared with that of the wild type parent E at 37 C. The production of infectious Ets-4 virus was moderately inhibited at this temperature and strongly inhibited at 42 C, whereas the wild type reproduced equally well at both temperatures. At 37 C, the mutant produced excessive amounts of poorly infectious 18S and 28S species of viral RNA compared with the parent and nearly normal amounts of infectious 46S RNA. Based on recent evidence by other workers, our data suggest that a rate-limiting step occurred at the presumed conversion of 28S RNA to 46S RNA.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0828736
Entities
People
- Arthur Brown
- Eugene Zebovitz